human fiia Search Results


monoclonal antibody directed to human fii  (Nordic BioSite)
90
Nordic BioSite monoclonal antibody directed to human fii
<t>Human</t> <t>FII-specific</t> antibody titers in pre- and posttreatment samples from wild-type and transgenic animals from line E (a) and line H (b), measured by ELISA and expressed as optical density (OD). Results were obtained from the screening assay where all samples were diluted 1:100. One transgenic male mouse from line E died during the study. Mean OD values are indicated by horizontal bars. Cut-off value was calculated to 0.438, values above this cutoff point were regarded as positive. Wt, wild type; Tg, transgenic.
Monoclonal Antibody Directed To Human Fii, supplied by Nordic BioSite, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+fiia/monoclonal+antibody+directed+to+human+fii/pmc03594975-55-12-18
Average 90 stars, based on 1 article reviews
monoclonal antibody directed to human fii - by Bioz Stars, 2026-10
90/100 stars
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mucor sp microsporum gypseum fiii  (ATCC)
94
ATCC mucor sp microsporum gypseum fiii
<t>Human</t> <t>FII-specific</t> antibody titers in pre- and posttreatment samples from wild-type and transgenic animals from line E (a) and line H (b), measured by ELISA and expressed as optical density (OD). Results were obtained from the screening assay where all samples were diluted 1:100. One transgenic male mouse from line E died during the study. Mean OD values are indicated by horizontal bars. Cut-off value was calculated to 0.438, values above this cutoff point were regarded as positive. Wt, wild type; Tg, transgenic.
Mucor Sp Microsporum Gypseum Fiii, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+fiia/Microsporum+gypseum/pm24937366__np401020a_si_001-78-28-43
Average 94 stars, based on 1 article reviews
mucor sp microsporum gypseum fiii - by Bioz Stars, 2026-10
94/100 stars
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innovin recombinant human thromboplastin  (Siemens AG)
90
Siemens AG innovin recombinant human thromboplastin
<t>Human</t> <t>FII-specific</t> antibody titers in pre- and posttreatment samples from wild-type and transgenic animals from line E (a) and line H (b), measured by ELISA and expressed as optical density (OD). Results were obtained from the screening assay where all samples were diluted 1:100. One transgenic male mouse from line E died during the study. Mean OD values are indicated by horizontal bars. Cut-off value was calculated to 0.438, values above this cutoff point were regarded as positive. Wt, wild type; Tg, transgenic.
Innovin Recombinant Human Thromboplastin, supplied by Siemens AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+fiia/pt+innovin/10__1309_slash_ajcps6ow6dynognh-49-6-10
Average 90 stars, based on 1 article reviews
innovin recombinant human thromboplastin - by Bioz Stars, 2026-10
90/100 stars
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monoclonal antibody directed to human fx  (Green Mountain Antibodies)
90
Green Mountain Antibodies monoclonal antibody directed to human fx
<t>Human</t> <t>FII-specific</t> antibody titers in pre- and posttreatment samples from wild-type and transgenic animals from line E (a) and line H (b), measured by ELISA and expressed as optical density (OD). Results were obtained from the screening assay where all samples were diluted 1:100. One transgenic male mouse from line E died during the study. Mean OD values are indicated by horizontal bars. Cut-off value was calculated to 0.438, values above this cutoff point were regarded as positive. Wt, wild type; Tg, transgenic.
Monoclonal Antibody Directed To Human Fx, supplied by Green Mountain Antibodies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+fiia/monoclonal+antibody+r8b12/pmc03594975-55-12-39
Average 90 stars, based on 1 article reviews
monoclonal antibody directed to human fx - by Bioz Stars, 2026-10
90/100 stars
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recombinant human fii  (AstraZeneca ltd)
90
AstraZeneca ltd recombinant human fii
<t>Human</t> <t>FII-specific</t> antibody titers in pre- and posttreatment samples from wild-type and transgenic animals from line E (a) and line H (b), measured by ELISA and expressed as optical density (OD). Results were obtained from the screening assay where all samples were diluted 1:100. One transgenic male mouse from line E died during the study. Mean OD values are indicated by horizontal bars. Cut-off value was calculated to 0.438, values above this cutoff point were regarded as positive. Wt, wild type; Tg, transgenic.
Recombinant Human Fii, supplied by AstraZeneca ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+fiia/recombinant+human+fii/pm25944555-58-24-30
Average 90 stars, based on 1 article reviews
recombinant human fii - by Bioz Stars, 2026-10
90/100 stars
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innovance at/ bcs-xp assay  (Siemens AG)
90
Siemens AG innovance at/ bcs-xp assay
<t>Human</t> <t>FII-specific</t> antibody titers in pre- and posttreatment samples from wild-type and transgenic animals from line E (a) and line H (b), measured by ELISA and expressed as optical density (OD). Results were obtained from the screening assay where all samples were diluted 1:100. One transgenic male mouse from line E died during the study. Mean OD values are indicated by horizontal bars. Cut-off value was calculated to 0.438, values above this cutoff point were regarded as positive. Wt, wild type; Tg, transgenic.
Innovance At/ Bcs Xp Assay, supplied by Siemens AG, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+fiia/innovance+at+assay/pmc12175104-116-8-31
Average 90 stars, based on 1 article reviews
innovance at/ bcs-xp assay - by Bioz Stars, 2026-10
90/100 stars
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pan fgfr inhibitor  (Tocris)
93
Tocris pan fgfr inhibitor
Fig. 4. FGF23 regulates TNAP through the FGFR3-ERK pathway. (A–D) mRNA abundance of TNAP and inorganic phosphate concentration in cell culture supernatant of differentiated calvarial osteoblasts isolated from WT animals after treatment with rFGF23 alone or in combination with a pan <t>FGFR</t> inhibitor <t>(A),</t> <t>FGFR1</t> inhibitor (B), FGFR3 inhibitor (C), or ERK1/2 inhibitor (D). (E) Dose-response curves for sodium-chloride cotransporter (NCC) mRNA expression in isolated segments of distal renal tubules and of TNAP mRNA expression in differentiated calvarial osteoblasts, 24 hours after treatment with different doses of rFGF23. Each data point is the mean SEM of 4 experimental samples in A–D and 3 to 6 samples per dose in E. p < 0.05 versus vehicle.
Pan Fgfr Inhibitor, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+fiia/FIIN+1+hydrochloride/pm26235988-64-66-71
Average 93 stars, based on 1 article reviews
pan fgfr inhibitor - by Bioz Stars, 2026-10
93/100 stars
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human α-thrombin (=fiia)  (Haemochrom Diagnostica)
90
Haemochrom Diagnostica human α-thrombin (=fiia)
Fig. 4. FGF23 regulates TNAP through the FGFR3-ERK pathway. (A–D) mRNA abundance of TNAP and inorganic phosphate concentration in cell culture supernatant of differentiated calvarial osteoblasts isolated from WT animals after treatment with rFGF23 alone or in combination with a pan <t>FGFR</t> inhibitor <t>(A),</t> <t>FGFR1</t> inhibitor (B), FGFR3 inhibitor (C), or ERK1/2 inhibitor (D). (E) Dose-response curves for sodium-chloride cotransporter (NCC) mRNA expression in isolated segments of distal renal tubules and of TNAP mRNA expression in differentiated calvarial osteoblasts, 24 hours after treatment with different doses of rFGF23. Each data point is the mean SEM of 4 experimental samples in A–D and 3 to 6 samples per dose in E. p < 0.05 versus vehicle.
Human α Thrombin (=Fiia), supplied by Haemochrom Diagnostica, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+fiia/human+antithrombin+41220/us09493472-913-25-45
Average 90 stars, based on 1 article reviews
human α-thrombin (=fiia) - by Bioz Stars, 2026-10
90/100 stars
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fiin 1 hydrochloride  (Tocris)
93
Tocris fiin 1 hydrochloride
Fig. 4. FGF23 regulates TNAP through the FGFR3-ERK pathway. (A–D) mRNA abundance of TNAP and inorganic phosphate concentration in cell culture supernatant of differentiated calvarial osteoblasts isolated from WT animals after treatment with rFGF23 alone or in combination with a pan <t>FGFR</t> inhibitor <t>(A),</t> <t>FGFR1</t> inhibitor (B), FGFR3 inhibitor (C), or ERK1/2 inhibitor (D). (E) Dose-response curves for sodium-chloride cotransporter (NCC) mRNA expression in isolated segments of distal renal tubules and of TNAP mRNA expression in differentiated calvarial osteoblasts, 24 hours after treatment with different doses of rFGF23. Each data point is the mean SEM of 4 experimental samples in A–D and 3 to 6 samples per dose in E. p < 0.05 versus vehicle.
Fiin 1 Hydrochloride, supplied by Tocris, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+fiia/FIIN+1+hydrochloride/pm25446536-56-55-58
Average 93 stars, based on 1 article reviews
fiin 1 hydrochloride - by Bioz Stars, 2026-10
93/100 stars
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candida maltosa sbug 700 candida albicans atcc 90028 candida krusei atcc 90878 aspergillus fumigatus microsporum canis fiii  (ATCC)
93
ATCC candida maltosa sbug 700 candida albicans atcc 90028 candida krusei atcc 90878 aspergillus fumigatus microsporum canis fiii
Fig. 4. FGF23 regulates TNAP through the FGFR3-ERK pathway. (A–D) mRNA abundance of TNAP and inorganic phosphate concentration in cell culture supernatant of differentiated calvarial osteoblasts isolated from WT animals after treatment with rFGF23 alone or in combination with a pan <t>FGFR</t> inhibitor <t>(A),</t> <t>FGFR1</t> inhibitor (B), FGFR3 inhibitor (C), or ERK1/2 inhibitor (D). (E) Dose-response curves for sodium-chloride cotransporter (NCC) mRNA expression in isolated segments of distal renal tubules and of TNAP mRNA expression in differentiated calvarial osteoblasts, 24 hours after treatment with different doses of rFGF23. Each data point is the mean SEM of 4 experimental samples in A–D and 3 to 6 samples per dose in E. p < 0.05 versus vehicle.
Candida Maltosa Sbug 700 Candida Albicans Atcc 90028 Candida Krusei Atcc 90878 Aspergillus Fumigatus Microsporum Canis Fiii, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+fiia/Candida+maltosa/pm24937366__np401020a_si_001-78-1-7
Average 93 stars, based on 1 article reviews
candida maltosa sbug 700 candida albicans atcc 90028 candida krusei atcc 90878 aspergillus fumigatus microsporum canis fiii - by Bioz Stars, 2026-10
93/100 stars
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anti fii  (Santa Cruz Biotechnology)
86
Santa Cruz Biotechnology anti fii
Fig. 4. FGF23 regulates TNAP through the FGFR3-ERK pathway. (A–D) mRNA abundance of TNAP and inorganic phosphate concentration in cell culture supernatant of differentiated calvarial osteoblasts isolated from WT animals after treatment with rFGF23 alone or in combination with a pan <t>FGFR</t> inhibitor <t>(A),</t> <t>FGFR1</t> inhibitor (B), FGFR3 inhibitor (C), or ERK1/2 inhibitor (D). (E) Dose-response curves for sodium-chloride cotransporter (NCC) mRNA expression in isolated segments of distal renal tubules and of TNAP mRNA expression in differentiated calvarial osteoblasts, 24 hours after treatment with different doses of rFGF23. Each data point is the mean SEM of 4 experimental samples in A–D and 3 to 6 samples per dose in E. p < 0.05 versus vehicle.
Anti Fii, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+fiia/Prothrombin+Antibody/pmc05173032-191-14-6
Average 86 stars, based on 1 article reviews
anti fii - by Bioz Stars, 2026-10
86/100 stars
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collagen vi  (Biosynth Carbosynth)
94
Biosynth Carbosynth collagen vi
Fig. 4. FGF23 regulates TNAP through the FGFR3-ERK pathway. (A–D) mRNA abundance of TNAP and inorganic phosphate concentration in cell culture supernatant of differentiated calvarial osteoblasts isolated from WT animals after treatment with rFGF23 alone or in combination with a pan <t>FGFR</t> inhibitor <t>(A),</t> <t>FGFR1</t> inhibitor (B), FGFR3 inhibitor (C), or ERK1/2 inhibitor (D). (E) Dose-response curves for sodium-chloride cotransporter (NCC) mRNA expression in isolated segments of distal renal tubules and of TNAP mRNA expression in differentiated calvarial osteoblasts, 24 hours after treatment with different doses of rFGF23. Each data point is the mean SEM of 4 experimental samples in A–D and 3 to 6 samples per dose in E. p < 0.05 versus vehicle.
Collagen Vi, supplied by Biosynth Carbosynth, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/human+fiia/Collagen+Type+VI+antibody/pm18927478-60-41-43
Average 94 stars, based on 1 article reviews
collagen vi - by Bioz Stars, 2026-10
94/100 stars
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Image Search Results


Human FII-specific antibody titers in pre- and posttreatment samples from wild-type and transgenic animals from line E (a) and line H (b), measured by ELISA and expressed as optical density (OD). Results were obtained from the screening assay where all samples were diluted 1:100. One transgenic male mouse from line E died during the study. Mean OD values are indicated by horizontal bars. Cut-off value was calculated to 0.438, values above this cutoff point were regarded as positive. Wt, wild type; Tg, transgenic.

Journal: Journal of Pharmaceutical Sciences

Article Title: A Triple-Transgenic Immunotolerant Mouse Model

doi: 10.1002/jps.23447

Figure Lengend Snippet: Human FII-specific antibody titers in pre- and posttreatment samples from wild-type and transgenic animals from line E (a) and line H (b), measured by ELISA and expressed as optical density (OD). Results were obtained from the screening assay where all samples were diluted 1:100. One transgenic male mouse from line E died during the study. Mean OD values are indicated by horizontal bars. Cut-off value was calculated to 0.438, values above this cutoff point were regarded as positive. Wt, wild type; Tg, transgenic.

Article Snippet: Nunc Maxisorp plates were coated with 100 μL/well monoclonal antibody directed to human FII (0.33–2 μg/mL, product P9115-04, Nordic Biosite, Täby, Sweden), human FVII (2 μg/mL product 2282, American Diagnostica, Pfungstadt, Germany) or human FX (2 μg/mL product GMA-520, Green Mountain Antibodies, Burlington, Vermomt), diluted in carbonate coating buffer (product C3041, Sigma Aldrich, Stockholm, Sweden).

Techniques: Transgenic Assay, Enzyme-linked Immunosorbent Assay, Screening Assay

Statistical Comparison of the Specific Antibody Response Between Samples from Wild-Type (Wt) and Transgenic (Tg) Mice Before and After Treatment with a Combination of Recombinant Human FII, FVII, and FX

Journal: Journal of Pharmaceutical Sciences

Article Title: A Triple-Transgenic Immunotolerant Mouse Model

doi: 10.1002/jps.23447

Figure Lengend Snippet: Statistical Comparison of the Specific Antibody Response Between Samples from Wild-Type (Wt) and Transgenic (Tg) Mice Before and After Treatment with a Combination of Recombinant Human FII, FVII, and FX

Article Snippet: Nunc Maxisorp plates were coated with 100 μL/well monoclonal antibody directed to human FII (0.33–2 μg/mL, product P9115-04, Nordic Biosite, Täby, Sweden), human FVII (2 μg/mL product 2282, American Diagnostica, Pfungstadt, Germany) or human FX (2 μg/mL product GMA-520, Green Mountain Antibodies, Burlington, Vermomt), diluted in carbonate coating buffer (product C3041, Sigma Aldrich, Stockholm, Sweden).

Techniques: Comparison, Transgenic Assay, Recombinant

Statistical Comparison of Values Above Cut-Point, Between Genders Within Respective Line, and Between Wild-Type (Wt) and Transgenic (Tg) Mice Following a Combination Treatment of Recombinant Human FII, FVII, and FX

Journal: Journal of Pharmaceutical Sciences

Article Title: A Triple-Transgenic Immunotolerant Mouse Model

doi: 10.1002/jps.23447

Figure Lengend Snippet: Statistical Comparison of Values Above Cut-Point, Between Genders Within Respective Line, and Between Wild-Type (Wt) and Transgenic (Tg) Mice Following a Combination Treatment of Recombinant Human FII, FVII, and FX

Article Snippet: Nunc Maxisorp plates were coated with 100 μL/well monoclonal antibody directed to human FII (0.33–2 μg/mL, product P9115-04, Nordic Biosite, Täby, Sweden), human FVII (2 μg/mL product 2282, American Diagnostica, Pfungstadt, Germany) or human FX (2 μg/mL product GMA-520, Green Mountain Antibodies, Burlington, Vermomt), diluted in carbonate coating buffer (product C3041, Sigma Aldrich, Stockholm, Sweden).

Techniques: Comparison, Transgenic Assay, Recombinant

Human FII-specific antibody titers in pre- and posttreatment samples from wild-type and transgenic animals from line E (a) and line H (b), measured by ELISA and expressed as optical density (OD). Results were obtained from the screening assay where all samples were diluted 1:100. One transgenic male mouse from line E died during the study. Mean OD values are indicated by horizontal bars. Cut-off value was calculated to 0.438, values above this cutoff point were regarded as positive. Wt, wild type; Tg, transgenic.

Journal: Journal of Pharmaceutical Sciences

Article Title: A Triple-Transgenic Immunotolerant Mouse Model

doi: 10.1002/jps.23447

Figure Lengend Snippet: Human FII-specific antibody titers in pre- and posttreatment samples from wild-type and transgenic animals from line E (a) and line H (b), measured by ELISA and expressed as optical density (OD). Results were obtained from the screening assay where all samples were diluted 1:100. One transgenic male mouse from line E died during the study. Mean OD values are indicated by horizontal bars. Cut-off value was calculated to 0.438, values above this cutoff point were regarded as positive. Wt, wild type; Tg, transgenic.

Article Snippet: Nunc Maxisorp plates were coated with 100 μL/well monoclonal antibody directed to human FII (0.33–2 μg/mL, product P9115-04, Nordic Biosite, Täby, Sweden), human FVII (2 μg/mL product 2282, American Diagnostica, Pfungstadt, Germany) or human FX (2 μg/mL product GMA-520, Green Mountain Antibodies, Burlington, Vermomt), diluted in carbonate coating buffer (product C3041, Sigma Aldrich, Stockholm, Sweden).

Techniques: Transgenic Assay, Enzyme-linked Immunosorbent Assay, Screening Assay

Statistical Comparison of the Specific Antibody Response Between Samples from Wild-Type (Wt) and Transgenic (Tg) Mice Before and After Treatment with a Combination of Recombinant Human FII, FVII, and FX

Journal: Journal of Pharmaceutical Sciences

Article Title: A Triple-Transgenic Immunotolerant Mouse Model

doi: 10.1002/jps.23447

Figure Lengend Snippet: Statistical Comparison of the Specific Antibody Response Between Samples from Wild-Type (Wt) and Transgenic (Tg) Mice Before and After Treatment with a Combination of Recombinant Human FII, FVII, and FX

Article Snippet: Nunc Maxisorp plates were coated with 100 μL/well monoclonal antibody directed to human FII (0.33–2 μg/mL, product P9115-04, Nordic Biosite, Täby, Sweden), human FVII (2 μg/mL product 2282, American Diagnostica, Pfungstadt, Germany) or human FX (2 μg/mL product GMA-520, Green Mountain Antibodies, Burlington, Vermomt), diluted in carbonate coating buffer (product C3041, Sigma Aldrich, Stockholm, Sweden).

Techniques: Comparison, Transgenic Assay, Recombinant

Statistical Comparison of Values Above Cut-Point, Between Genders Within Respective Line, and Between Wild-Type (Wt) and Transgenic (Tg) Mice Following a Combination Treatment of Recombinant Human FII, FVII, and FX

Journal: Journal of Pharmaceutical Sciences

Article Title: A Triple-Transgenic Immunotolerant Mouse Model

doi: 10.1002/jps.23447

Figure Lengend Snippet: Statistical Comparison of Values Above Cut-Point, Between Genders Within Respective Line, and Between Wild-Type (Wt) and Transgenic (Tg) Mice Following a Combination Treatment of Recombinant Human FII, FVII, and FX

Article Snippet: Nunc Maxisorp plates were coated with 100 μL/well monoclonal antibody directed to human FII (0.33–2 μg/mL, product P9115-04, Nordic Biosite, Täby, Sweden), human FVII (2 μg/mL product 2282, American Diagnostica, Pfungstadt, Germany) or human FX (2 μg/mL product GMA-520, Green Mountain Antibodies, Burlington, Vermomt), diluted in carbonate coating buffer (product C3041, Sigma Aldrich, Stockholm, Sweden).

Techniques: Comparison, Transgenic Assay, Recombinant

Fig. 4. FGF23 regulates TNAP through the FGFR3-ERK pathway. (A–D) mRNA abundance of TNAP and inorganic phosphate concentration in cell culture supernatant of differentiated calvarial osteoblasts isolated from WT animals after treatment with rFGF23 alone or in combination with a pan FGFR inhibitor (A), FGFR1 inhibitor (B), FGFR3 inhibitor (C), or ERK1/2 inhibitor (D). (E) Dose-response curves for sodium-chloride cotransporter (NCC) mRNA expression in isolated segments of distal renal tubules and of TNAP mRNA expression in differentiated calvarial osteoblasts, 24 hours after treatment with different doses of rFGF23. Each data point is the mean SEM of 4 experimental samples in A–D and 3 to 6 samples per dose in E. p < 0.05 versus vehicle.

Journal: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research

Article Title: FGF23 Regulates Bone Mineralization in a 1,25(OH)2 D3 and Klotho-Independent Manner.

doi: 10.1002/jbmr.2606

Figure Lengend Snippet: Fig. 4. FGF23 regulates TNAP through the FGFR3-ERK pathway. (A–D) mRNA abundance of TNAP and inorganic phosphate concentration in cell culture supernatant of differentiated calvarial osteoblasts isolated from WT animals after treatment with rFGF23 alone or in combination with a pan FGFR inhibitor (A), FGFR1 inhibitor (B), FGFR3 inhibitor (C), or ERK1/2 inhibitor (D). (E) Dose-response curves for sodium-chloride cotransporter (NCC) mRNA expression in isolated segments of distal renal tubules and of TNAP mRNA expression in differentiated calvarial osteoblasts, 24 hours after treatment with different doses of rFGF23. Each data point is the mean SEM of 4 experimental samples in A–D and 3 to 6 samples per dose in E. p < 0.05 versus vehicle.

Article Snippet: After osteoblastic differentiation (50 mg/mL ascorbic acid and 10 mM b-glycerophosphate) for 6 days, cells were treated with 10-7 M or 10-8 M of 1,25(OH)2D3, 10 or 100 ng/mL of recombinant human FGF23 R176/179Q (rFGF23, kindly provided by Amgen Inc., Thousand Oaks, CA, USA), 20 ng/mL rat anti-FGF23 antibody (kindly provided by Amgen Inc.), 10 nM FGFR1 inhibitor PD173074 (Sigma, St. Louis, MO, USA), 120 nM pan FGFR inhibitor (FIIN1 hydrochloride, Tocris Bioscience, Bristol, UK), 5 ng/mL ERK inhibitor PD184352 (Sigma), and 25 nM FGFR3 inhibitor PD173074 (Sigma) for 24 hours.

Techniques: Concentration Assay, Cell Culture, Isolation, Expressing